简繁切换
Cloud Zoom small image
Anti-TROP2 Chimeric antibody [A5C5] 嵌合抗体 | 货号: HA600011

￥ 1500.00
当前销售数量:0
更多精品特惠 > >
 折扣:0折
本单还可以继续购买
数量: 库存 1500 件
立即购买
加入购物车
  • 详情
  • 特别提示
  • 评价
  • 产品描述Tumor-associated calcium signal transducer 2, also known as Trop-2 and as epithelial glycoprotein-1 antigen (EGP-1), is a protein that in humans is encoded by the TACSTD2 gene. This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. This antigen is the target of sacituzumab govitecan, an antibody-drug conjugate. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells. This human monoclonal chimeric antibody has been engineered from the mouse monoclonal parent antibody. By necessity, some mouse sequence is retained as part of the variable domain. When multiplexing with other mouse-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
  • 产品名称Anti-TROP2 Chimeric antibody [A5C5]
  • 分子量35.7 kDa
  • 种属反应性Human
  • 验证应用ELISA,FC
  • 抗体类型嵌合抗体
  • 免疫原Recombinant protein within Human TROP2 aa27-274 (Extracellular) in HEK293 cell.
  • 偶联Non-conjugated
  • RRIDAB_3071629

  • 性能

  • 形态Liquid
  • 浓度1 mg/ml.
  • 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液1*PBS (pH7.4).
  • 亚型IgG1
  • 纯化方式Protein G affinity purified.
  • 亚细胞定位Membrane.
  • 数据链接SwissProt: P09758 Human
  • 其它名称
    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    more

  • 应用

    ELISA: 1:5,000-1:20,000
    FC: 1:100-1:200

  • Fig1: The binding activity of HA600011 with recombinant TROP2 protein.
    Immobilized recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600011) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of HA600011 is 6.958 ng/ml.

    Fig2: Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with HA600011 at 1/200 dilution (red) compared with Purified human IgG, Isotype Control (blue). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody.

  • 背景文献

  • 1. Anthony Simms et al. TROP-2 expression in papillary thyroid carcinoma: Potential Diagnostic Utility. Diagn Cytopathol. 2016 Jan;44(1):26-31.
特别推荐